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Fe 3+ can bind to transferrin (TF) in serum and is subsequently taken up by the TF receptor 1 (TfR1), which is encoded by TFRC on the cell membrane [52]

For instance, compared with normal cells, tumor cells show an increased demand for iron to synthesize DNA and complete self-proliferation [44, 45]

doi: 10.1038/srep29872 (2016)

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In brief, up to 1 million cells were resuspended in a total of 180 L freshly prepared FACS Buffer (PBS, 1% FSC, 2 mM EDTA)

GSMe is usually a much weaker inhibitor in the HEDS assay or in assays with GSSR substrates 11,14,17 , suggesting that the competition between GSH and GSMe in the roGFP2 measurements occurred at the 1st and not the 2nd glutathione interaction site