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Probst DT, Sookochoff MF, Harris-Hayes M, Prather H, Lipsey KL, Cheng AL

4 Materials and methods 4.1 Cell culture and in vivo experiments 4.1.1 Cell lines Four human NB cell lines were used in this paper: Be(2)-C (MNA), IMR5-/75-shMYCN (29) (MNA, doxycycline-inducible shRNA targeting MYCN, resulting in MYCN downregulation), IMR-32 (MNA), and SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (non-MNA, doxycycline-inducible transgene, resulting in MYCN overexpression)

[3] Iron is not absorbed as well when taken with calcium or calcium-containing foods like dairy, or with foods high in oxalates (such as spinach, kale, beets, rhubarb, chocolate, nuts, and wheat bran)

This can last for a few days but should fade