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For LCP crystallization, the protein loaded onto the Ni-NTA column was washed with 8 column volume (CV) basis buffer containing 0.1% DDM and 0.01% CHS and 15 CV basis buffer containing 50 mM imidazole and 0.1% DDM and 0.01% CHS

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Note that guidance is included in all arthrography radiological supervision and interpretation codes and so 77002 should not be added to those procedures