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Five micrometer sections were cut using a fully automated Leica RM 2155 rotary microtome (Leica Microsystem, Nussloch, Germany) from the paraffin blocks

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Methylation regulates expression in relation to imprinted genes through a pivotal process, which causes an occurrence of the allele pertaining to maternal or paternal side

PARK2 mutant mice present with pathological behavior disrupted glucose metabolism and pathological gene expression in the midbrain PD patients with PARK2 mutations have upregulated -oxidation and dysregulated glucose metabolism 14,15