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Q-PCR Q-PCR experiments for tRNA Gly , specifically, cDNA synthesis was performed with 500 ng of total RNA, following the protocol accompanying the PrimeScript RT-PCR Kit (Takara, Dalian, China) with the CFX96TM Real-Time System (BIO-RAD, California, USA), particularly necessary to point out that the reverse transcription primers should chose Random 6 mers for reverse transcription of total tRNA

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