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Epithalon and Anti-Aging Cellular Effects In vitro studies on skin fibroblasts have investigated Epithalons potential impact on cell aging, proliferation, and extracellular matrix regulation.(5) Key reported effects: Decreased expression of MMP-9 , a matrix metalloproteinase that tends to increase with age Increased expression of Ki-67 and CD98hc , markers associated with cell proliferation and nutrient transport Inhibition of Caspase-3 , a central executioner protease in apoptosis These findings suggest that Epithalon may: Promote cellular regeneration and proliferation Suppress caspase-dependent apoptosis that rises during in vitro aging Support structural and functional maintenance of dermal cells 4

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The paramagnetic probe, S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSL), was used to label the cysteine residue 46 of p53 TAD 67 , where 300 M MTSL-labeled p53 TAD was added to 150 M 15 N-labeled FOXO4 FHD