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Description

M.McLane-SvobodaA

cDNA was synthesized using Hifair II 1st Strand cDNA Synthesis SuperMix (Yeasen, Cat

doi:10.1155/2015/487160 VeDA

In fact, SUMO cannot be used in eukaryotes because there is already too much SUMO protease around, but it is convenient when used with purified protein since the enzyme cleaves the SUMO tag in the same manner as it would have in the context of a cell