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Biomass from 1 L of culture was resuspended in 20 mL TES buffer (0.5 M sucrose, 0.5 mM EDTA, and 0.2 M Tris-HCl pH 8.0) for dehydration at 4 C for 0.5 h, followed by rehydration by dilution with 40 mL of ice-cold MilliQ H 2 O at 4 C for 1 h