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Preparation of colon and peripheral blood single-cell suspensions Colon biopsies were thawed for 2 min with gentle agitation in a 37 o C water bath, transferred to a gentleMACS C Tube (Miltenyi Biotec), washed twice with basal media containing 10 M Y-27632, and then incubated in 5 mL digestion buffer (basal media), 10 M Y-27632, 600 U/mL Collagenase IV (Worthington cat

Adv Med Sci 63:6878 Stern BR, Solioz M, Krewski D, Aggett P, Aw TC, Baker S, Crump K, Dourson M et al (2007) Copper and human health: biochemistry, genetics, and strategies for modeling dose-response relationships

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Supplementation with 50,000 IU vitamin D every 2 weeks over 12 weeks has been shown to reduce high-sensitivity C-reactive protein levels, increase total antioxidant capacity, improve the total cholesterol/HDL ratio and relieve pelvic pain (127)