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The cysteine content was measured by the colorimetric method using reduced phosphotungstic acid to produce tungsten blue ( The dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) activity was assayed according to Ma and Cheng (2003) by using the assay kit DHAR-1-W and MDHAR-1-W (Suzhou Comin Biotechnology, Suzhou, China), respectively

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The L6 cells were differentiated with 2% Horse serum containing media for 72 hr post which the cells were incubated with Madhugrit (10-100 g/ml) for 24 hr