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Additionally, cell viability was evaluated using live/dead staining with a Calcein-AM/PI double staining kit and observed under a fluorescence microscope (Olympus, Japan)

Combined with the prediction of CELLO, WoLF PSORT, and TargetP subcellular localization servers, plant GPXs were clustered into five groups: mitochondria and chloroplast localized GPXs, cytoplasm localized GPXs, cytoplasm/extracellular/plasma membrane localized GPXs, cytoplasm/mitochondria/chloroplast localized GPXs, and cytoplasm/extracellular/nucleus localized GPXs (Supplementary Table 4)

Mycobacterium bovis BCG cell wall and lipopolysaccharide induce a novel gene, BIGM103, encoding a 7-TM protein: identification of a new protein family having Zn-transporter and Zn-metalloprotease signatures

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