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Glucose influx into the system is fixed at 100, because this analysis is intended to reveal relative metabolic flux distribution relative to total glucose uptake rate

However, determining the precise volume of the pre-filling buffer, based on the estimated elution volume before chromatography, adds complexity and reduces the robustness of the process during large-scale manufacturing due to the critical need for controlled mixing to ensure rapid and uniform homogenization (Shukla et al., 2007

Many proteins are very large and composed of hundreds and hundreds of amino acids

Conversely, iron deficiency might alter warfarin metabolization through cytochrome P450, a heme-containing enzyme [350,351]

Due to limited neutrophil yield from each blood draw, the entire span of dose-response curve was covered from two different donors (indicated by color code), with several overlapping conditions to ensure the trend and main observations are consistent across donors

Energy metabolism and fertility: a balance preserved for female health