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Health , 23 , 539-559, FAO, WHO (2002) Human Vitamin and Mineral Requirements

doi: 10.1038/s41556-020-0461-8 88 SongXZhuSChenPHouWWenQLiuJet al

M.van den BergH.BastA

The extent of proteolysis at the conclusion of the digestion process was little impacted by the procedure (liquid vs spray-dried), with 50.8% for F2 compared to 56.8% for F1 and 52.9% for F3, with 5% of the variation among these formulations

It was worth mentioning that the catalytic activity of CDs increased significantly with the increase in irradiation time (Figure 4)

Proteins were extracted from 5 to 50 ml of exponentially growing suspension culture (OD 600 =0.81.2) by centrifugation and grinding with glass beads (5 min, IKAVibrax-VXR, Janke und Kunkel) at 4 C in 150300 l Native lysis buffer (50 mM Na-HEPES (pH 7.5)