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Sections were mounted in VECTASHIELD Antifade Mounting Medium and observed under a KEYENCE BZ-X700 fluorescence microscope equipped with a digital camera and a Tile-Scan program (Figure 2e)

These findings highlight the necessity for further investigations into the impact of berberine on liver functions across a broader spectrum of fish species

The observed variability in response across different species underscores the imperative for additional research to elucidate the underlying mechanisms of berberines effects