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In the eye field, 24 hours after retinal ischemiareperfusion, key indicators of ferroptosis, such as lipid peroxidation and the downregulation of GPX4, rise in tandem with apoptosis markers, including cleaved caspase-3 and TUNEL-positive cells (132)

To quantify the number of LC3-positive puncta and AO-red fluorescence intensity, 30 cells were recorded in randomly selected fields of view by fluorescence microscopy, and the average fluorescence values were analyzed by Image J

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Beyond its anti-ferroptotic role, AB4 exerts multimodal effectsparticularly in combating inflammationthrough this RAGE-Nrf2 axis