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After sugar depletion in the initial medium containing 4% (v/v) molasses and 5% (v/v) CSL, a feeding solution of 50% (v/v) molasses and 25% (v/v) CSL was continuously added to the bioreactor

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then, at the point of 24 h, the next application was given (isoprenaline given twice, at 0 h and 24 h) for re-infarct induction