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The samples were subjected to SDS-PAGE and Western blotting, detected by rabbit polyclonal A1CT antibody generated in Lefkowitz lab 58 (1:5000) and HRP-conjugated ECL Rabbit IgG (NA9340, Amersham, RRID:AB_772191) (1:5000) for arr1 and HRP-conjugated Anti-6X His-tag antibody (1:2000) (ab1187, Abcam, RRID: AB_298652) for SH3

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Although, these genes play a major role in detoxification of host derived RNI, they were not found to be essential for virulence