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For spot growth assays, yeast cultures were grown overnight in liquid culture and exponentially growing cells serially diluted to 5,000/1,000/200/40/8 cells per l (1:5 steps)

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The cocktail and mosaic group mounted the broadest NAb responses, while the NAb responses induced by the monovalent AMS3a E2E1-NP group were skewed towards the autologous AMS3a virus (Fig

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However, adverse effects are often reported, most of which relate to skeletal muscle function