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Infection at the injection site is rare but possible if sterile techniques aren't followed properly

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Cells were first washed with PBS, fixed with methanol and acetic acid (3:1, v/v) for 1 hr, stained with crystal violet (0.1%) for 1 hr, and then washed with water

When the experiments were repeated at 100 nM APOE concentrations, tau phosphorylation was not affected

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