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2020, Feb, 11;323(6):548

Occludin, the first identified tight junction protein, 29,30 has a dual role in the intestinal barrier

This provides comparison points for evaluating progress

What I can tell you is what I observe

Membrane fluidity depends on the temperature, the ratio of saturated/PUFA fatty acids, the presence of lipid rafts, and the proportion of cholesterol present at the membrane [31]