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The method is based on the assumption, that the binding of the (pro)drug occurs mainly inside the cells and that estimation of C u,cell indicates the overall unbound drug concentration in the brain intracellular compartment without specifying the distribution to the specific parenchymal cells or subcellular structures

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However, there should be a delicate balance between ROS generation and antioxidant defense to avoid an increased oxidative stress, which could be detrimental to sperm (141, 142)

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