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The peptide is often supplied in a lyophilized, freeze-dried form and needs mixing with sterile water or bacteriostatic water before use in research protocols

Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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The only way to know if it's working for you is to track baseline metrics and compare

The parthenolide derivative ACT001 synergizes with low doses of L-DOPA to improve MPTP-induced Parkinsons disease in mice