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W., Donohue, S

found that glutamine blockade enhanced the anti-tumor effects of the anti-PD-1 therapy compared with antiPD-1 therapy alone

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Neutrophils were removed from co-culture with cancer cells after 3 h of incubation at 37 degrees, washed, counted and 50,000 neutrophils re-suspended in 50 l 1X PBS, and placed in a black 96 well plate (Corning 3904), with each condition performed in triplicate