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DEGs of LPS vs Control were intersected with ferroptosis gene regulators including driver, suppressor, marker, and unclassified regulator in FerrDb database ( Statistical analysis All statistical analyses of scRNA-seq data were performed using R 3.5.2 ( The in vitro and in vivo experiments were repeated independently, and similar results were obtained at least three times

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J Invest Dermatol 145(6):1345-1360 e2

FIGURE 8 3.8 GHK-Cu inhibited the expression of RORt in DSS-induced colitis Western blot analysis showed that treatment with GHK-Cu significantly reduced DSS-induced RORt expression (Figure 9), implying Th17 cell inhibition contributes to its therapeutic effect