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Glutathione synthesis is ATP-consuming and depends on the availability of the amino acids cysteine, glutamine and glycine and requires two subsequent reactions, in which -glutamylcysteine synthetase (-ECS or GSH1, EC 6.3.2.2) first catalyzes the formation of -glutamylcysteine (-EC) from cysteine and glutamine and glutathione synthetase (GSHS or GSHS2, EC 6.3.2.3), then catalyzes the formation of glutathione from -EC and glycine

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Montagne A, Nation DA, Sagare AP, Barisano G, Sweeney MD, Chakhoyan A, Pachicano M, Joe E, Nelson AR, DOrazio LM, Buennagel DP, Harrington MG, Benzinger TLS, Fagan AM, Ringman JM, Schneider LS, Morris JC, Reiman EM, Caselli RJ, Chui HC, Tcw J, Chen Y, Pa J, Conti PS, Law M, Toga AW, Zlokovic BV (2020) APOE4 leads to blood-brain barrier dysfunction predicting cognitive decline