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Image analysis and base calling were performed using Illuminas Real Time Analysis v1.18.66.3 software, followed by demultiplexing of indexed reads and generation of FASTQ files using Illuminas bcl2fastq Conversion Software v1.8.4

The cells were analysed using an EVOS FL microscope (ThermoFisher Scientific) and processed in ImageJ2 software (NIH) and the BMG FLUOstar Omega Microplate Reader

Sources Biological and Pharmaceutical Bulletin, Limonoids: overview of significant bioactive triterpenes distributed in plants kingdom, 2006 British Journal of Clinical Pharmacology, Grapefruit juice-drug interactions, 1998 Checklist for Staying Healthy, p

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Product Specifications Key Features Multiple-Dose Access: The bacteriostatic preservative inhibits the growth of most potentially contaminating bacteria, allowing the vial to be used for multiple draws over a 28-day period (once opened)

Animal models In vivo experiments were performed with 24 month-old (young group) and 79 month-old (old group) C57 Bl/6 N wild-type mice of both sexes or with age-matched female APP23xPS45 mice expressing the APP Swedish mutation (670/671) and the G384A mutation in the presenilin 1 (PS1) gene under the Thy-1 promotor 14