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These developmental factors, combined with genetic predispositions or environmental triggers, make babies and children especially susceptible to eczema

Understanding how it compares to alternatives helps optimize protocol selection

The fine gauge minimizes the puncture hole in the rubber stopper (extending the useful life of the vial) and creates less tissue trauma during subcutaneous administration

Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

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