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Additionally, PF is more prevalent among the elderly population, typically presenting between the ages of 5070 years with a higher prevalence in men than women [4]

Transcriptomic and unbiased metabolomic modeling in ndufs2 -/- larvae uncovered a host of dysregulated pathways and genes that may be downstream cellular effects of primary CI deficiency that significantly impact animal survival and health and thereby warrant investigation for potential therapeutic manipulation

2 In 1985, the methylenedioxy and methoxy derivatives of the amphetamines were placed in Schedule 1 of the restricted drugs list in the United States, 1 and they are classified similarly in Canada (Schedule III of the Controlled Drugs and Substances Act) and the United Kingdom (Class A under the Misuse of Drugs Act)

After centrifugation, PBS was used to resuspend the cells

All assays were performed in triplicate on independent days to ensure reproducibility, and results are reported as mean standard deviation