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After collagen polymerization (1 hour at 5% CO 2 and 37C), each well was filled with 73 l of culture media and left overnight in the incubator for cell acclimatization

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The next day, cells were washed and incubated for 90 min at room temperature with Alexa Fluor-conjugated secondary antibodies (anti-mouse Alexa Fluor 555, Invitrogen

Phase Low-moderate Daily dose 20-50 mcg/day Site Trained muscle, post-workout Notes Often used in research interest in localized hypertrophy signaling

Glyphosate does not appear in this table because there is no well-established link between glyphosate and any specific unexplained clinical presentation in humans at typical population-level exposures