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Windler E, Havel R

Sample mounting, staining and imaging: ultra-thin sections (85 nm) obtained with a Leica Ultracut UCT ultramicrotome (Leica Microsystems) were mounted on copper grids with parlodion-carbon support film, placed sideways on a droplet of 2% uranyl acetate in H 2 O for 30 min, immersed repeatedly in H 2 O, air dried overnight, placed sideways on a droplet of Satos lead staining solution for 20 min, immersed repeatedly in H 2 O, air dried for 30 min and imaged at 80 kV and 50,000 magnification

DNA binding proteins that can be used in these methods DNA binding proteins which can be used in these methods include, but are not limited to, transcriptional regulators, enzymes involved in DNA replication (e.g., recA) and reassembly (&/or one or more additional directed evolution methods described herein), and proteins that serve structural functions on DNA (e.g., histones, protamines)

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