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DNA concentration was quantified using a Qubit 3.0 fluorometer, and the purified DNA was subsequently used for qPCR

doi: 10.1182/bloodadvances.2020003597 43 AlmeidaARMNetoJLCachuchoAEuzebioMMengXKimRet al

The results indicated that the overall prevalence of zinc deficiency in this cohort was 23.2%, with 72.4% of participants exhibiting dietary zinc intake levels below the estimated average requirement (EAR)