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Tape stripping has been utilised as a pre-treatment to enhance medication penetration and as an analytical technique to evaluate the depth and uniformity of drug permeation into the skin

One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations

Diabetes Obes Metab 2017;19:52436.PubMed 64

It is constitutively expressed at a high level in different mammalian cell lines and normal tissues, but the specific transcription factors involved in the regulation of its expression have not yet been completely identified

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Among them, GCL expression and cysteine concentration are the key factors for GSH synthesis 61