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Glucose-6-Phosphate Dehydrogenase Deficiency The importance of this enzyme for red cell integrity was first recognized following the observation that some African-American soldiers taking the antimalarial drug primaquine would develop acute hemolytic anemia with hemoglobinuria

Hepatology (2013) 57:136677

Then, from 12 noon until 3 pm we assume that the input is (1.75)A corresponding to lunch, followed by three hours of fasting and then an input of (3.25)A for three hours corresponding to dinner

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing