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Creation of the mutant library by error-prone PCR The error-prone PCR of chppk gene was performed by TIANDZ error-prone PCR kit ( The plasmid pXMJ-19-tac- Ch PPK was used as template, and the F and R primers are seen Additional file 1 and were applied to obtain the mutant fragments of Ch PPK from error-prone PCR, accompanied by the changes of MgCl 2 and MnCl 2 [25]

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