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Fatty acid metabolism Increasing evidence suggests that fatty acids play crucial roles in cancer cachexia via diverse mechanisms, including energy provision, metabolic modulation, anti-inflammatory and immune regulation and intestinal barrier maintenance (Fig

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[DOI] [PMC free article] [PubMed] [Google Scholar] 62.Huang H., Liu N., Guo H., Liao S., Li X., Yang C., Liu S., Song W., Liu C., Guan L

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Immunoblotting was then performed with voltage-dependent anion channel 2 (VDAC2) antibody (#9412, Cell Signaling Technology, Beverly, MA, USA), the translocase of the outer mitochondrial membrane 20 (TOM20) antibody, (#42406, Cell Signaling Technology), total OXPHOS Rodent WB antibody cocktail (ab110413, Abcam), anti-PGC-1 polyclonal antibody (#AB3242, EMD Millipore Corporation, CA, USA), anti-NRF1 antibody (sc-33771, Santa Cruz Biotechnology, Inc.), anti- TFAM antibody (sc-23588, Santa Cruz Biotechnology, Inc.), anti-PPAR monoclonal antibody (Santa Cruz Biotechnology, Inc.), anti-ATF5 rabbit monoclonal antibody (Abcam), anti-CHOP mouse monoclonal antibody (#2895, Cell Signaling Technology), and anti-actin antibody (A2066, Sigma-Aldrich, St