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ROS, especially mitochondrial ROS (mtROS), serve as potent activators by releasing danger-associated molecular patterns (DAMPs), while the antioxidant N-acetylcysteine (NAC) mitigates this activation [43]

In the United States, the Food and Drug Administration (FDA) classifies ashwagandha as a dietary supplement, meaning it is not approved for the treatment or prevention of specific diseases and is subject to less stringent pre-market review compared with pharmaceuticals [225]

Increasing cellular nad+ Protects hepatocytes against palmitate-induced lipotoxicity by preventing parp-1 inhibition and the mtorc1-P300 pathway activation

Transgenic overexpression of Sry sensitizes mice to APAP-induced liver damage and apoptosis, whereas hepatocyte-specific knockout of Sry protects against these conditions We previously reported the generation of hepatocyte-specific Sry transgenic mice ( Sry- TG) overexpressing Sry in hepatocytes under the control of the albumin (Alb) gene promoter [17], as well as hepatocyte-specific Sry knockout mice ( Sry HC ) generated by crossbreeding SRY flox mice ( Sry fl/fl ) with Alb-Cre transgenic mice [18]