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Immunity 39 , 10191031 (2013)

In the presence of metal ions, they chelate and produce OH radicals by decreasing H 2 O 2 (Fig

Glutathione helps keep the thiols of proteins and other compounds in a reduced state and is also involved in the metabolism of other antioxidants, such as vitamin C (ascorbate) and vitamin E (alpha-tocopherol) (17

Svendsen et al

Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

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