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Rakoff-Nahoum, S., Paglino, J., Eslami-Varzaneh, F., Edberg, S

After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS

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If your dog has a diagnosed heart condition or a breed predisposition, treat supplementation as a coordinated plan with your veterinarian or cardiologist

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