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Isolation of primary hepatocytes Primary mouse hepatocytes were obtained using a modified version of a two-step collagenase perfusion technique described by Seglen [28]

LT: Investigation, Writing review & editing, Funding acquisition, Writing original draft

References Abdallah C, Dumas-Gaudot E, Renaut J, Sergeant K (2012) Gel-based and gel-free quantitative proteomics approaches at a glance

Next, we evaluated the role of SIRT1 in protection against NIHL by exposing both SIRT1 CKO and SIRT1 flox/flox mice to noise at 108 dB SPL for 2 h and assessing hearing thresholds after 14 days (Fig

Journal of Medicinal Plants Research , 5(8), 1272-1279

Acknowledgments We would like to thank the native English speaking scientists of Elixigen Company (Huntington Beach, California) for editing our manuscript