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To test if the inducible degradation of Vma2 protein affects vacuolar acidification, we fused the C terminus of Pho8, a vacuolar-resident protein with the C terminus facing the lumen, with a pH-sensitive super ecliptic pHluorin (SEP) protein that displays increased fluorescence with higher pH levels 27

N., & University of Maryland Center of Excellence in Regulatory Science and Innovation (M-CERSI)

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Each of these ingredients helps the body turn fat into energy and are also powerful antioxidants

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The base is usually 500-1000 ml of either 0.9% sodium chloride (normal saline) or Lactated Ringer's solution , which provides immediate hydration and electrolyte replacement