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( Reference Dutta, Seirafi and Halpin 39 ) , although riboflavin deficiency alone did not affect the activities of GPx and GR and increased the content of glutathione in the liver, ethanol administration (which can exacerbate oxidative stress) significantly decreased the activities of these enzymes and the content of glutathione in the liver of riboflavin-deficient rats compared with those in the liver of the riboflavin-sufficient control rats

It is necessary to test female rats throughout the estrus cycle since they are more variable than male rats and include both sexes increases variability [19]

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