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Lactyl-CoA analysis and LCMS quantification Lactyl-CoA was quantified by LCMS/MS as previously described 15

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Second, cells also need to be able to recycle the oxidized glutathione back to its reduced state

PMID: 39746501 Chem Biol Interact Aristolochic acid I induced mitochondrial Ca2+ accumulation triggers the production of MitoROS and activates Src/FAK pathway in hepatocellular carcinoma cells

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Pulidindi K, Ahuja K