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For SPE, 100 L of 3- tert -butylanisole (2 mM in absolute ethanol, 99.9 %, VWR, Darmstadt, Germany), 10 L of the internal standard 4-methoxy-2-methyl-2-butanethiol (11.2 M in ethanol), and 20 L of ETP (99 %, Merck, Darmstadt, Germany) were added to 50 mL of the sample

doi: 10.3390/cells10082032 162 AmoozgarZKloepperJRenJTayREKazerSWKinerEet al

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For most consumer-facing research-vial contexts, the simpler answer is: keep lyophilized powder frozen or refrigerated before mixing, keep reconstituted solution refrigerated after mixing, and avoid freeze-thaw cycles

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