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PLOS ONE, 12(11), e0187962

Quantification of intracellular itaconate Raw264.7 cells were plated at 2 10 6 per well in 6-well plates overnight

To further inspect single cells, we switched from bulk cultures to a high-throughput small-volume cultivation in 96-well plates where the change in pigmentation and production rates of EV can be observed in single cells