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After adding 1 mM DTT, 10 mM MgCl 2, and 2 mM ATP to remove contamination of heat shock proteins, the eluted target protein was concentrated with a 50 kDa cut-off concentrator (Millipore) and further purified by the Superose 6 increase 10/300 GL column (Cytiva) running in a buffer containing 20 mM HEPES pH 8.0, 150 mM NaCl, 0.025% DDM, and 0.0025% CHS

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Homocysteine and inborn errors of metabolism Often, what is known about biochemical pathways originates from observations of the natural experiments that are inborn errors of metabolism

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The full peptide name The sequence GEPPPGKPADDAGLV The expected molecular mass of the peptide The observed identity result The chromatographic method and result How arginate is defined for that material The measured arginine content Any other counterions detected The net peptide assay The water and residual-solvent results where relevant The batch or lot number The analysis date and issuing laboratory What Does 99% HPLC Purity Not Prove

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