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These results were validated using fluorescent miscroscopy and immunocytochemical staining ( Figure 4B ), which showed that CB-839 induced the accumulation of LC3 puncta in HG-3 and MEC-1 cells

[PubMed: 29625838] 27

Pasquali, M

When pretreating the cells with N -ethylmaleimide (NEM, 20 mM), a trapping reagent of thiol species, for 0.5 h, the signals from the cytoplasmic regions are decreased in both green and yellow channels (Figure 7DF)

The DNA methylation patterns that occur throughout the genome are referred to as the methylome, and DNA methylation within the promoter region to the first exon of a gene generally correlates with decreased gene expression (Brenet et al., 1998