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My daughter was diagnosed with B12 deficiency last year and her B12 test results said her level was so low it was unable to be read

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Immunoprecipitates were pelleted, washed twice with cell lysis buffer, washed with PBS and then eluted in 100 mM glycine-HCl, pH 2.5, by incubation for 1 h on ice

To be able to analyse RLR activation by HCV, we therefore used Huh7 cells overexpressing MAVS containing the C508R mutation preventing NS3/4A-mediated cleavage (MAVScr) 45