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As achieving calciumfree conditions is generally challenging and can create artifacts in proteoliposomes, another approach was employed to eliminate the calcium binding site by mutagenesis

This outer shell is comprised of two layers as illustrated in Figure 1B: a cytoplasmic membrane (RBC cm ) and a spectrin-based cytoskeleton (Liu et al., 1987)

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Moreover, in transfected cells or in vitro assays using artificial membranes, the NEC stimulates uncontrolled vesicle formation, which is absent in infected cells 48,49,50

Microbiome of Penaeus vannamei larvae and potential biomarkers associated with high and low survival in shrimp hatchery tanks affected by acute hepatopancreatic necrosis disease

In vitro release studies showed sustained release for over two weeks, best fitted by zero-order kinetics