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These findings indicate Day 30 of liver organoids as the optimal stage for drug metabolism and toxicological investigations, providing a proteomic framework that enhances their utility of iHLOs as physiologically relevant liver models

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This proposal was supported by the essentially perfect correlation for binding affinity differences between the selected pair of compounds obtained by rigorous free energy perturbation calculations by Gutierrez-de-Terans group (Vanga et al., 2018)