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Statistical analyses Data were typically expressed as the mean of at least three biological replicates standard error of the mean (SEM)

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Adding 2mL creates a 5mg/mL concentration that gives better precision at lower doses but requires split draws at 8mg and above

The grid squares on the edge of the grid were gently pierced by a sharp tweezer, so that the broken grid squares could be used as a reference point to find axon cut sites

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